. 2018 Apr 15;177:112–123. doi: 10.1016/j.jprot.2018.02.015

Table 1.

Proteins detected in quadriceps tissue containing redox sensitive Cys residues including the relative quantification of oxidative state of susceptible Cys residues and changes in the redox ratio with age. Relative protein abundance is included and proteins in bold change significantly with age.

Accession	Protein	Adult:Old	Significance (–10logP)	Peptide	Cysteine	Adult Red/Ox	Old Red/Ox	Change with age
P62259	14-3-3 Protein epsilon (Ywhae)	1.00:1.16	6.06	LICCDILDVLDK	97/98^*	7.69	22.47	2.92
P61982	14-3-3 Protein gamma (Ywhag)	1.00:1.04	6.62	NCSETQYESK	112	14.21	36.02	2.53
Q60597	2-Oxoglutarate dehydrogenase (Ogdh)	1.00:1.12	2.15	DVVVDLVCYR	507	0.53	0.71	1.35
P47857	6-Phosphofructokinase (Pfkm)	1.00:0.70	8.36	GITNLCVIGGDGSLTGADTFR	114	5.63	9.68	1.72
				LPLMECVQVTK	351	8.65	15.08	1.74
				CNENYTTDFIFNLYSEEGK	631	5.22	21.04	4.03
				IFANTPDSGCVLGMR	709	3.37	14.03	4.16
Q99KI0	Aconitate hydratase (Aco2)	1.00:1.00	1.85	VAVPSTIHCDHLIEAQVGGEK	126	61.41	26.17	0.43
				VGLIGSCTNSSYEDMGR	385	0.66	1.12	1.70
				DVGGIVLANACGPCIGQWDR	448/451^*	0.13	0.33	2.55
				CTTDHISAAGPWLK	592	19.33	22.92	1.19
O88990	Actin, alpha skeletal muscle (Acta1)	1.00:2.82	99.81	LCYVALDFENEMATAASSSSLEK	218	4.65	6.16	1.33
O88990	Actin, alpha skeletal muscle (Acta1)			CDIDIR	286	4.36	6.32	1.45
P45376	Aldose reductase (Akr1b1)	1.00:0.74	15.33	LIEYCHSK	200	0.71	0.81	1.14
P05201	Aspartate aminotransferase (Got1)	1.00:1.10	4.28	INMCGLTTK	391	3.54	7.45	2.10
P05202	Aspartate aminotransferase (Got2)	1.00:1.13	4.92	EYLPIGGLAEFCK	106	5.36	10.69	1.99
				TCGFDFSGALEDISK	187	4.31	3.99	0.93
				VGAFTVVCK	295	0.16	0.06	0.36
Q9DCX2	ATP synthase dubunit d mitochondrial (Atp5h)	1.00:1.27	8.20	SCAEFVSGSQLR	101	51.73	37.60	0.73
P45591	Cofilin-2 (Cfl2)	1.00:1.07	5.60	LLPLNDCR	80	1.90	13.71	7.21
P07310	Creatine kinase M-type (Ckm)	1.00:1.14	3.01	GYTLPPHCSR	146	6.40	18.06	2.82
P07310	Creatine kinase M-type (Ckm)			FCVGLQK	254	11.50	28.41	2.47
Q6P8J7	Creatine kinase S-type (Ckmt2)	1.00:0.95	5.14	SEVELVQIVIDGVNYLVDCEK	397	3.60	7.42	2.06
Q9CZ13	Cytochrome b-c1 complex subunit 1 (Uqcrc1)	1.00:0.92	9.70	LCTSATESEVTR	380	1.96	1.60	0.82
Q9DB77	Cytochrome b-c1 complex subunit 2 (Uqcrc2)	1.00:1.09	6.20	NALANPLYCPDYR	192	0.02	0.02	0.95
O08749	Dihydrolipoyl dehydrogenase (Dld)	1.00:1.99	8.53	NETLGGTCLNVGCIPSK	80/85^*	71.21	307.42	4.32
Q99LC5	Electron transfer flavoprotein subunit alpha (Etfa)	1.00:0.85	12.77	LGGEVSCLVAGTK	53	15.40	5.53	0.36
P58252	Elongation factor 2 (Eef2)	1.00:0.59	25.73	ETVSEESNVLCLSK	591	2.86	6.59	2.30
P58252	Elongation factor 2 (Eef2)			EGALCEENMR	693	14.47	17.27	1.19
P17182	Alpha-enolase (Enoa)	1.00:1.32	1.46	FGANAILGVSLAVCK	119	2.14	8.36	3.91
P17182	Alpha-enolase (Enoa)			TGAPCR	398	4.18	8.93	2.14
P21550	Beta-enolase (Enob)	1.00:0.89	8.60	SGETEDTFIADLVVGLCTGQIK	389	3.60	6.42	1.79
P05064	Fructose bisphosphate aldolase A (Aldoa)	1.00:1.04	3.97	YASICQQNGIVPIVEPEILPDGDHDLK	178	0.53	2.08	3.91
				CQYVTEK	202	2.50	6.56	2.62
				ALANSLACQGK	339	5.77	11.37	1.97
P06745	Glucose 6-phosphate isomerase (Gpi)	1.00:1.25	6.57	MIPCDFLIPVQTQHPIR	404	0.48	2.89	6.06
P16858	Glyceraldehyde-3-phosphate dehydrogenase (Gapdh)	1.00:1.02	8.18	IVSNASCTTNCLAPLAK	150/154^*	127.86	554.21	4.33
P16858	Glyceraldehyde-3-phosphate dehydrogenase (Gapdh)			VPTPNVSVVDLTCR	245	17.90	26.74	1.49
Q9WUB3	Glycogen phosphorylase (Pygm)	1.00:0.98	10.01	ICGGWQMEEADDWLR	172	6.47	10.09	1.56
				TCAYTNHTVLPEALER	373	4.81	10.53	2.19
				WLVLCNPGLAEVIAER	496	14.05	18.31	1.30
				QLLNCLHIITLYNR	581	30.97	12.73	0.41
P63017	Heat shock cognate 71 kDa protein (Hspa8)	1.00:0.79	11.10	VCNPIITK	603	10.88	8.79	0.81
P02089	Hemoglobin subunit beta-2 (Hbb-b2)	1.00:1.54	5.99	GTFASLSELHCDK	94	100.34	91.44	0.91
P06151	l-Lactate dehydrogenase A chain (Ldha)	1.00:0.71	23.73	DYCVTANSK	84	13.62	20.01	1.47
P06151	l-Lactate dehydrogenase A chain (Ldha)			VIGSGCNLDSAR	163	1.33	4.29	3.23
P14152	Malate dehydrogenase (Mdh1)	1.00:1.06	6.17	VIVVGNPANTNCLTASK	137	15.57	35.51	2.28
P14152	Malate dehydrogenase (Mdh1)			ENFSCLTR	154	0.94	6.36	6.80
P08249	Malate dehydrogenase (Mdh2)	1.00:0.76	10.78	GCDVVVIPAGVPR	93	0.69	2.52	3.63
				TIIPLISQCTPK	212	22.06	82.92	3.76
				EGVVECSFVQSK	275	2.44	9.84	4.02
				ETECTYFSTPLLLGK	285	4.35	9.62	2.21
Q5XKE0	Myosin-binding protein C fast-type (Mybpc2)	1.00:0.81	12.24	IFSENICGLSDSPGVSK	1007	48.64	77.79	1.60
P97457	Myosin regulatory light chain 2 (Mylpf)	1.00:1.63	39.47	QFLEELLTTQCDR	128	16.04	22.38	1.40
P97457	Myosin regulatory light chain 2 (Mylpf)			NICYVITHGDAK	157	7.32	13.91	1.90
P15532	Nucleoside diphosphate kinase A (Nme1)	1.00:0.59	12.77	GDFCIQVGR	109	14.89	14.54	0.98
P17742	Peptidyl prolyl cis-trans isomerase (PpiA)	1.00:0.90	2.84	IIPGFMCQGGDFTR	62	2.52	5.76	2.29
P09411	Phosphoglycerate kinase 1 (Pgk1)	1.00:0.59	23.68	GCITIIGGGDTATCCAK	367/379/380^*	47.50	95.40	2.01
O70250	Phosphoglycerate mutase 2 (Pgam2)	1.00:0.71	30.92	FCGWFDAELSEK	23	7.19	9.20	1.28
O70250	Phosphoglycerate mutase 2 (Pgam2)			IEFDICYTSVLK	55	1.31	1.11	0.85
Q9D0F9	Phosphoglucomutase 1 (Pgm1)	1.00:0.75	14.98	TIEEYAICPDLK	160	15.52	16.76	1.08
Q9D0F9	Phosphoglucomutase 1 (Pgm1)			LSLCGEESFGTGSDHIR	374	3.25	8.42	2.59
Q99LX0	Protein DJ-1 (park7)	1.00:0.81	6.52	DPVQCSR	46	8.50	17.53	2.06
Q9QYG0	Protein NDRG2 (Ndrg2)	1.00:0.83	9.86	CPVMLVVGDQAPHEDAVVECNSK	255/274^*	14.57	32.14	2.21
Q9D051	Pyruvate dehydrogenase E1 component subunit beta (Pdhb)	1.00:0.82	6.78	EGIECEVINLR	263	6.80	9.97	1.47
P52480	Pyruvate kinase isozymes M1/M2 (Pkm2)	1.00:0.98	3.86	NTGIICTIGPASR	49	12.06	17.11	1.42
				CDENILWLDYK	152	5.75	8.62	1.50
				GIFPVLCK	474	23.16	29.64	1.28
P07724	Serum albumin (Alb)	1.00:1.25	9.00	CSYDEHAK	58	2.95	2.78	0.94
P17751	Triosephosphate isomerase (Tpi1)	1.00:0.84	8.68	IAVAAQNCYK	117	0.63	2.40	3.80
				VSHALAEGLGVIACIGEK	177	9.15	11.75	1.28
				IIYGGSVTGATCK	268	7.29	13.17	1.81
P58771	Tropomyosin alpha-1 chain (Tpm1)	1.00:2.60	115.68	CAELEEELK	190	5.65	9.46	1.68
P58774	Tropomyosin beta chain (Tpm2)	1.00:2.04	84.11	CGDLEEELK	190	6.34	7.89	1.25
P13412	Troponin-1 fast skeletal muscle (Tnni2)	1.00:2.32	52.67	VCMDLR	134	6.79	10.27	1.51

Signifies that 2 or more Cys residues present in tryptic peptide.