Figure 2.

MiR-200a is up-regulated in AML-12 stimulated by ethanol.

a MTT assay was performed to assess the cytotoxicity of ethanol on AML-12 cells. The IC₅₀ is equal to 538.55±5.12 mM. b The apoptosis rate calculated by three independent Annexin V-FITC staining experent. c Annexin V-FITC staining represent the apoptosis rate caused by ethanol treatment. One representative experiment of the three independent experiments is demonstrated. d MiR-200a expression in ethanol-stimulated AML-12 was analyzed by qPCR. The result are shown as relative expression against control expression without treatment. e MiR-200a expression in acetaldehyde-stimulated AML-12 was analyzed by qPCR. The result are shown as relative expression against control expression without treatment. * P <0.05,** P <0.01 versus control.