Figure 6.

Up-regulated ZEB2 expression contributes to the inhibition of hepatocyte apoptosis (a) ZEB2 successful over-expression was affirmed by western blot and qPCR. The result are shown as relative expression against control expression without treatment. Data were presented as mean ±SD from three independent experiments. (b) AML-12 cells were transfected with ZEB2 or vector for 24 h alone or in combination with ethanol. The stained apoptosis cells with TUNEL were visualized with a fluorescence microscope (original magnification, ×20). (c) The expression of Bax and Bcl-2 were determined by western blot in AML-12 cells. The results are shown as relative expression against control expression without treatment. Data were presented as mean ± SD from three independent experiments. * P < 0.05, ** P < 0.01 versus control. # P< 0.05 versus ethanol treatment group, ns, not significant versus ethanol treatment group.