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. 2018 Jan 21;17(2):200–215. doi: 10.1080/15384101.2017.1407890

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 3. — Condensin depletion disorganizes the rDNA loop at 25 ºC. (A) Strains FM1009 (OsTIR1 NET1:GFP ycs4:aid), FM2055 (NET1:GFP ycs4:aid) and FM2150 (OsTIR1 NET1:GFP smc2:aid*) were spotted on YPD plates with and without 1 mM IAA and incubated for 3 days at 25 ºC. (B) Strain FM1009 was blocked in metaphase (Nz x 3h) at 25 ºC with or without the simultaneous addition of 1 mM IAA. Samples were taken for each condition and immediately visualized under the microscope without further processing. (C) Strain FM2150 was treated like in B. Samples were frozen 24 h at -20 ºC before staining with DAPI and visualized under the microscope. White scale bars represent 5 μm. BF, bright field; Nz, nocodazole; IAA, 3-indolacetic acid.