Fig. 3.

Construction andin vitrocharacterization of nsp2TF/nsp2N-deficient mutants. (A) Nucleotide sequences in the regions of mutations created in nsp2TF/nsp2N-deficient mutants. KO1, knockout mutant 1 (premature termination codons in TF ORF); KO2, knockout mutant 2 (premature termination codon and disrupted frameshift signal). Mutated nucleotides are shown in red. (B) Radioimmunoprecipitation detection of nsp2-related proteins in virus infected cells. MAb 140-68 was used to recognize the common N-terminal PLP2 domain. The truncated nsp2TF in vKO1-infected cells is indicated with a red arrow. (C) Multiple-step virus growth curve. Each data point shown represents the mean value from duplicates, and error bars show SEM.