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. 2018 Apr;517:164–176. doi: 10.1016/j.virol.2017.12.017

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© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig S1 — Expression of nsp2-related proteins in HEK-293T cell. HEK-293T cells seeded in a 24-well plate were transfected with a plasmid (0.5 μg) expressing each of nsp2-related protein. At 24 hpi, immunofluorescence assay was performed to evaluate protein expression. The α-FLAG mAb was used to detect the expression of FLAG-tagged proteins. Rabbit pAb (α-TF) specifically recognizing the unique C-terminal domain of nsp2TF was used to detect the expression of full-length nsp2TF (Li et al., 2014), while rabbit pAb (α-nsp2) specifically recognizing the nsp2C-terminus was used to detect the expression of full-length nsp2 (Guo et al., 2016). Cell nucleus was stained with DAPI.