Skip to main content
. Author manuscript; available in PMC: 2018 May 21.
Published in final edited form as: Nat Immunol. 2017 Nov 21;19(1):85–97. doi: 10.1038/s41590-017-0001-2

Figure 5. Transcriptional heterogeneity of CB lympho-myeloid progenitor cells from single cell RNA-sequencing.

Figure 5

(a) Experimental scheme used to combine single cell functional analysis, single cell RNA-sequencing and single cell qRT-PCR based on flow cytometric index data. (b) Heatmap showing clustering of single LMPP, GMP and MLP using the 55 most highly and variably expressed genes between clusters. Heatmap shows clustering from one of two donors analyzed. Data from the other donor are in Supplementary Fig. 5d. Log-normalized gene expression (rows) for each single cell (columns) is shown. (c-d) PCA plot colored by cell type (c) or cluster membership (d).