Figure 8.

Facilitation and asynchronous release under physiological conditions in wild-type and Syt7 KO animals. Experiments were performed at 35°C in 1.5 mm external Ca²⁺. A, Paired-pulse facilitation in representative PCs for wild-type and Syt7 KO animals. B, Summarized paired-pulse facilitation curves for wild-types (n = 5) and Syt7 KOs (n = 5). Single (solid) and double (dashed) exponential fits are shown. Asynchronous release was examined in wild-type and Syt7 KO animals (C–F). C, Average evoked current by single stimuli in a representative wild-type SC. D, Single stimuli evoked delayed events during single trials for cells shown in C. Synchronous component of the EPSC and stimulus artifact are blanked. E, Raster plot of quantal events. F, The time course and amplitude of quantal events evoked by single stimuli summarized across cells in wild-type (n = 9) and Syt7 KOs (n = 8). Data are mean ± SEM. For some points error bars are occluded by markers.