Table 1. Primer sequences used in qRT-PCR.
| Gene | Accession No. | Primer Sequence (5’-3’) | Size (bp) | Tm (°C) | Efficiency (%) | R2 |
|---|---|---|---|---|---|---|
| RPS7 | AB669190 | F: CGCCAACAAACAGAAGAGGC | 219 | 55.5 | 93.2 | 0.997 |
| R: CGCCCCGTAAGCTTCTTGTA | ||||||
| RPS13 | GQ222274 | F: ACTGACTGCTGATGATGTGAAGG | 77 | 57.5 | 106.3 | 0.996 |
| R: TGACACCGATTTGGGAGGG | ||||||
| 18S | FJ875999. | F: CGGCGACGCATCTTTCAA | 85 | 56.8 | 105.7 | 0.997 |
| R: TTCCCCGTTACCCGTGACA | ||||||
| GAPDH | HM055756 | F: AAAATCTCCGTCCTCTCCGA | 140 | 55.8 | 109.9 | 0.990 |
| R: ACCTTCTTGGCACCACCCT | ||||||
| ACT | GQ856238 | F: AACTTCCCGACGGTCAAGTCAT | 168 | 60 | 104.6 | 0.996 |
| R: TGTTGGCGTACAAGTCCTTACG | ||||||
| TUB | EU100016 | F: CGGTAATGCCTGCTGGGAA | 118 | 54.3 | 94.0 | 0.998 |
| R: CTCGCTGAAGAAGGTGTTGAA | ||||||
| RPL32 | AB669190 | F: GTGAAAAAGCGGACGAAAAGA | 187 | 53 | 95.2 | 0.987 |
| R: GGAAACCATTGGGCAGCATA | ||||||
| EF-1α | KR869785 | F: CTCCACTGAGCCCCCATACA | 128 | 59.4 | 108.4 | 0.994 |
| R: CTCCGTGCCAGCCAGAAAT |