Table 2. Stability of reference genes in different developmental stages and insect tissues.
Condition | Rank | Δ Ct | BestKeeper | NormFinder | geNorm | ||||
---|---|---|---|---|---|---|---|---|---|
Gene | stability value | Gene | stability value | Gene | Stability value | Gene | Stability value | ||
Dev. stage | 1.00 | ACT | 1.29 | TUB | 0.43 | 18S | 0.46 | ACT | 0.53 |
2.00 | RPS13 | 1.30 | ACT | 0.73 | RPS13 | 0.46 | EF-1α | 0.53 | |
3.00 | EF1-α | 1.31 | RPS13 | 0.82 | ACT | 0.48 | TUB | 0.75 | |
4.00 | 18S | 1.31 | GAPDH | 0.83 | EF1-α | 0.53 | RPS13 | 0.98 | |
5.00 | TUB | 1.42 | 18S | 0.84 | TUB | 0.67 | 18S | 1.07 | |
6.00 | RPS7 | 1.73 | EF-1α | 1.10 | RPL32 | 1.11 | RPS7 | 1.28 | |
7.00 | RPL32 | 1.80 | RPL32 | 1.38 | GAPDH | 1.19 | RPL32 | 1.38 | |
8.00 | GAPDH | 1.91 | RPS7 | 1.67 | GAPDH | 1.18 | GAPDH | 1.51 | |
Tissues | 1.00 | TUB | 1.21 | TUB | 0.17 | RPS13 | 0.25 | TUB | 0.22 |
2.00 | RPS13 | 1.21 | EF1-α | 0.22 | TUB | 0.58 | EF1-α | 0.22 | |
3.00 | EF1-α | 1.26 | GAPDH | 0.54 | ACT | 0.66 | RPS13 | 0.81 | |
4.00 | ACT | 1.31 | RPS7 | 0.75 | EF1-α | 0.72 | ACT | 0.90 | |
5.00 | 18S | 1.45 | RPS13 | 0.80 | 18S | 0.97 | 18S | 0.97 | |
6.00 | RPS7 | 1.63 | ACT | 1.04 | RPS7 | 1.32 | RPS7 | 1.14 | |
7.00 | GAPDH | 1.73 | 18S | 1.06 | GAPDH | 1.54 | GAPDH | 1.23 | |
8.00 | RPS32 | 2.41 | RPL32 | 2.11 | RPS32 | 2.31 | RPS32 | 1.53 |