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. 2018 Apr 4;13(4):e0195096. doi: 10.1371/journal.pone.0195096

Table 2. Stability of reference genes in different developmental stages and insect tissues.

Condition Rank Δ Ct BestKeeper NormFinder geNorm
Gene stability value Gene stability value Gene Stability value Gene Stability value
Dev. stage 1.00 ACT 1.29 TUB 0.43 18S 0.46 ACT 0.53
2.00 RPS13 1.30 ACT 0.73 RPS13 0.46 EF-1α 0.53
3.00 EF1-α 1.31 RPS13 0.82 ACT 0.48 TUB 0.75
4.00 18S 1.31 GAPDH 0.83 EF1-α 0.53 RPS13 0.98
5.00 TUB 1.42 18S 0.84 TUB 0.67 18S 1.07
6.00 RPS7 1.73 EF-1α 1.10 RPL32 1.11 RPS7 1.28
7.00 RPL32 1.80 RPL32 1.38 GAPDH 1.19 RPL32 1.38
8.00 GAPDH 1.91 RPS7 1.67 GAPDH 1.18 GAPDH 1.51
Tissues 1.00 TUB 1.21 TUB 0.17 RPS13 0.25 TUB 0.22
2.00 RPS13 1.21 EF1-α 0.22 TUB 0.58 EF1-α 0.22
3.00 EF1-α 1.26 GAPDH 0.54 ACT 0.66 RPS13 0.81
4.00 ACT 1.31 RPS7 0.75 EF1-α 0.72 ACT 0.90
5.00 18S 1.45 RPS13 0.80 18S 0.97 18S 0.97
6.00 RPS7 1.63 ACT 1.04 RPS7 1.32 RPS7 1.14
7.00 GAPDH 1.73 18S 1.06 GAPDH 1.54 GAPDH 1.23
8.00 RPS32 2.41 RPL32 2.11 RPS32 2.31 RPS32 1.53