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| Experimental conditions | No. reference genes | Recommended reference genes |
|---|---|---|
| Developmental stages | 3 | ACT, EF1-α and TUB |
| Tissues | 3 | TUB, RPS13 and EF1-α |
| High temperature | 2 | RPS13 and 18S |
| Larval crowding | 3 | TUB, RPS13 and RPS7 |
| Pesticide exposure | 4 | RPS7, EF1-α, RPL32 and GAPDH |
