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. 2018 Apr 4;13(4):e0195212. doi: 10.1371/journal.pone.0195212

Fig 9. Hepatic injury and apoptosis in RGZ treated mice following reperfusion.

Fig 9

Analysis of hepatic injury showed no significant difference in the RGZ vs Control group for AST (14451±3518 vs 18231±3688U/L, p = 0.482), Inhibition of PPAR-γ with GW9662 increased further the levels of AST when compared with control group (A). Representative images of TUNEL immunohistochemistry showing a similar grade of apoptosis in RGZ, CTRL and RGZ+GW-9662 groups at 6hr post-reperfusion (B). TUNEL positive staining was found similar in RGZ vs control group at 6hr post-reperfusion (42±6 vs 48±3%, p = 0.442) (C). GW-9662 showed a reversal of the slight beneficial effect on apoptosis found in the RGZ group (C). Note: Staining was assessed with image software analysis. Abbreviations: AST-aspartate aminotransferase, RGZ-Rosiglitazone, CTRL-Control, TUNEL-Terminal deoxynucleotidyl transferase(TdT) dUTP Nick-End-Labeling. Five experiments (n = 5) per group per time point were performed. Results are shown as mean ± SEM, Mann-Whitney U test.