. 2018 Feb 8;176(4):2700–2719. doi: 10.1104/pp.17.01828

Table I. Summary of transgenic lines generated and used in this study.

Wild-Type and Transgenic Lines	Genetic Background and Cross	Transgene Information
Nb	N. benthamiana	Wild-type, no transgene
DCL1i	RNAi of DCL1 in Nb	pRNAi-DCL1, single copy, hemizygous
DCL2Ai	RNAi of DCL2 in Nb	pRNAi-DCL2, single copy, homozygous
DCL2Bi	RNAi of DCL2 in Nb	pRNAi-DCL2, single copy, homozygous
DCL3Ai	RNAi of DCL3 in Nb	pRNAi-DCL3, single copy, homozygous
DCL3Bi	RNAi of DCL3 in Nb	pRNAi-DCL3, single copy, homozygous
DCL4Ai	RNAi of DCL4 in Nb	pRNAi-DCL4, single copy, homozygous
DCL4Bi	RNAi of DCL4 in Nb	pRNAi-DCL4, single copy, homozygous
RDR6i (Gift from David Baulcombe)	RNAi of NbRDR6 in Nb	RDR6 hairpin, single copy, homozygous
DCL24i	Cross between DCL2Ai and DCL4Bi	pRNAi-DCL2, single copy, hemizygous
DCL24i	Cross between DCL2Ai and DCL4Bi	pRNAi-DCL4, single copy, hemizygous
16cGFP	GFP transgenic line in Nb	35S-GFP, single copy, homozygous
GfpRDR6i (Gift from David Baulcombe)	RNAi of RDR6 in 16cGFP	35S-GFP, single copy, homozygous
GfpRDR6i (Gift from David Baulcombe)	RNAi of RDR6 in 16cGFP	RDR6 hairpin, single copy, homozygous
GfpDCL1i	RNAi of DCL1 in 16cGFP	35S-GFP, single copy, homozygous
GfpDCL1i	RNAi of DCL1 in 16cGFP	pRNAi-DCL1, single copy, hemizygous
GfpDCL2Ai	Cross between 16cGFP and DCL2Ai	35S-GFP, single copy, hemizygous
GfpDCL2Bi	Cross between 16cGFP and DCL2Bi	pRNAi-DCL2, single copy, hemizygous
GfpDCL3Ai	Cross between 16cGFP and DCL3Ai	35S-GFP, single copy, hemizygous
GfpDCL3Bi	Cross between 16cGFP and DCL3Bi	pRNAi-DCL3, single copy, hemizygous
GfpDCL4Ai	Cross between 16cGFP and DCL4Ai	35S-GFP, single copy, hemizygous
GfpDCL4Bi	Cross between 16cGFP and DCL4Bi	pRNAi-DCL4, single copy, hemizygous
GfpDCL24Ai	Triple cross among 16c, DCL2Ai and DCL4Ai	35S-GFP, single copy, hemizygous
GfpDCL24Bi	Triple cross among 16c, DCL2Ai and DCL4Bi	pRNAi-DCL2, single copy, hemizygous
GfpDCL24Bi	Triple cross among 16c, DCL2Ai and DCL4Bi	pRNAi-DCL4, single copy, hemizygous
homGfpDCL2Ai	Self from GfpDCL2Ai	35S-GFP, single copy, homozygous
homGfpDCL2Ai	Self from GfpDCL2Ai	pRNAi-DCL2, single copy, homozygous
homGfpDCL3Bi	Self from GfpDCL3Bi	35S-GFP, single copy, homozygous
homGfpDCL3Bi	Self from GfpDCL3Bi	pRNAi-DCL3, single copy, homozygous
homGfpDCL4Ai	Self from GfpDCL4Ai	35S-GFP, single copy, homozygous
homGfpDCL4Ai	Self from GfpDCL4Ai	pRNAi-DCL4, single copy, homozygous
GfpDCL24Ci	Cross between homGfpDCL2Ai and homGfpDCL4Ai	35S-GFP, single copy, homozygous
		pRNAi-DCL2, single copy, hemizygous
		pRNAi-DCL4, single copy, hemizygous
Gfp	Cross between Nb and 16cGFP	35S-GFP, single copy, hemizygous