Figure 3.

BBX24 functionally mimics BBX21 when their C-terminal regions are interchanged. A, Immunoblot analysis of transgenic seedlings expressing GFP-BBX24, GFP-BBX21, GFP-BB24C21, and GFP-BB21C24 driven by the 35S promoter. Samples from 4-d-old dark-grown seedlings exposed to 75 μmol m⁻² s⁻¹ of white light were harvested and anti-GFP antibodies were used to visualize the fusion proteins. Upper and middle panels (labeled as GFP) represent a short and a long exposure, respectively. UGPase was used as loading control. B and C, Photograph of representative plants grown for 3 (B), 7 and 9 (C) weeks in long day conditions (16:8 light/dark) of 100 μmol m⁻² s⁻¹ white light.