Figure 6.

BBX21-specific function is not dependent on Motif 7. A, Schematic presentation of full-length BBX24 and BBX21 proteins and two chimeric versions. B, Yeast two-hybrid assay using HY5 as bait and BBX24, BBX21, BBM7_24, and BBM7M6_24 as prey. C, Hypocotyl measurements of 5-d-old seedlings grown in 100 μmol m⁻² s⁻¹ monochromatic red light. Error bars represent se, n ≥ 21. * and ** indicates P < 0.05 and P < 0.01, respectively, compared to wild type as determined by one-way ANOVA, P ≤ 0.05. B1, first B-box; B2, second B-box; 7, Motif 7; 6, Motif 6; SD2, media lacking Trp, Leu; SD4, media lacking Trp, Leu, His, Ura; 3-AT, addition of 1 μM 3-amino-1, 2,4-triazol to the growth media; VP, VP domain; WT, wild type.