Figure 9.

Membrane lipid composition and expression of genes involved in lipid recycling do not change in response to altered PCho or PEth content. A and B, Lipid molecular species from the triple KO mutant (A) or 35S:PPsPase1 #A overexpressing line (B) do not differ from their respective controls. KO and wild-type seedlings were grown in vitro for 8 d in low Pi. Extracts from wild-type and 35S:PPsPase1 #A shoots were obtained from plants grown hydroponically in high Pi conditions. Results are presented as the mean ± sd of five biological replicates. C and D, Knockout or overexpressing lines do not show altered regulation of the genes involved in lipid recycling during Pi starvation. C, Triple knockout and corresponding wild-type controls were grown in vitro for 8 d in low Pi. Wild-type control values were arbitrarily set to one and are represented here as a dotted line. D, Overexpressing lines for PPsPase1 (#A, solid light gray bar; #B, solid dark gray bar) and PECP1 (#A, striped light gray bar; #B, striped dark gray bar) were grown in vitro for 8 d in high Pi along with wild-type plants. Wild-type control values were arbitrarily set to one (dotted line). Results are presented as the median ± 95% confidence interval of independent biological replicates (REST randomization test, n = 2 or 3). SCAMP, ROC3, and GAPC1 were used as reference genes for the RT-qPCR. No significant differences were observed.