Phenotypic characterization of oft1 mutant lines. A, Inflorescence morphology of 6-week-old oft1 mutants and wild-type Col-0 controls. Blue arrowheads indicate improperly developed siliques. Bars = 1 cm. B, Representative images of fully developed siliques harvested from 6-week-old oft1 mutant or wild-type Col-0 control plants. The genotypes of siliques are indicated in the top right corner of each image. Bars = 2 mm. C, Quantification of 6-week-old Col-0 (black bar) and oft1 mutant (red bars) silique lengths. Data are means ± se (n = 23). One-way ANOVA indicated a significant difference between Col-0 and oft1 mutants (**, P < 0.01 by Tukey’s posthoc analysis). D, Fully developed siliques of the indicated genotypes were harvested from 6-week-old plants and cleared in 70% (v/v) ethanol as described in “Materials and Methods.” Representative siliques from each genotype are shown. E, Quantification of Col-0 (black bar) and oft1 mutants (red bars) seed set. Data are means ± se (n = 12–22 siliques). One-way ANOVA indicated a significant difference between Col-0 and oft1 mutants (**, P < 0.01 by Tukey’s posthoc analysis).