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. 2018 Feb 21;176(4):2804–2818. doi: 10.1104/pp.17.01577

Figure 6.

Figure 6.

Semi-in vivo penetration competition assay of oft1-3−/−; 11p::OFT1-GFP+/− pollen tubes. A, GFP and bright-field overlaid images depicting the bottom of an ms1 stigma displaying penetrating pollen tubes. Red arrowheads indicate nonfluorescent pollen tubes, and yellow arrowheads indicate fluorescent pollen tubes. B, Quantification of the percentage of fluorescent pollen tubes emerging from the TT at 4 HAP. Three independent oft1-3−/−;11p:OFT1-GFP+/− transgenic lines (red bars) were assessed. 9p::YFP+/− pollen tubes (black bar) served as a negative control. Data are means ± sd (n ≥ 7).