Table I. Segregation distortion analysis of oft1 mutant and complemented lines.
| Parents (♂ × ♀)a | #Rb | #Sb | Total | %R | TEc | χ2d | P |
|---|---|---|---|---|---|---|---|
| oft1-1+/− self | 258 | 247 | 505 | 51.1 | 1.04 | 38.5 | <0.00001 |
| oft1-2+/− self | 51 | 57 | 108 | 47.2 | 0.89 | 11.1 | 0.0009 |
| oft1-3+/− self | 369 | 328 | 697 | 52.9 | 1.13 | 45.2 | <0.00001 |
| Col-0 × oft1-1+/− | 57 | 52 | 109 | 52.3 | 1.10 | 0.11 | 0.74 |
| oft1-1+/− × Col-0 | 0 | 116 | 116 | 0 | 0 | 58 | <0.00001 |
| Col-0 × oft1-3+/− | 364 | 340 | 704 | 51.7 | 1.07 | 0.41 | 0.52 |
| oft1-3+/− × Col-0 | 1 | 1,871 | 1,872 | 0.05 | 5.3 × 10−4 | 934 | <0.00001 |
| Col-0 × oft1-3−/−; 11p::OFT1-GFP+/−e | 84 | 102 | 186 | 45.2 | 0.82 | 0.87 | 0.35 |
| oft1-3−/−; 11p::OFT1-GFP+/− × oft1-3−/−e | 494 | 0 | 494 | 0 | ND | 247 | <0.00001 |
| Col-0 × oft1-3+/−; 11p::OFT1-GFP+/−e | 161 | 158 | 319 | 50.5 | 1.02 | 0.014 | 0.91 |
| oft1-3+/−; 11p::OFT1-GFP+/− × Col-0e | 771 | 1,688 | 2,459 | 31.4 | 0.46 | 171.0 | <0.00001 |
a
The parent lines of each cross are indicated. Transgenic lines containing the 11p::OFT1-GFP construct are abbreviated as 11p::OFT1-GFP with the indicated genotype.
b
The total number of resistant (#R) and sensitive (#S) individuals in each cross F1 progeny are indicated. Transmission of alleles was scored as described in “Materials and Methods”: oft1-1 (kanamycin resistance), oft1-2 (PCR genotyping), oft1-3 (BASTA resistance), and 11p::OFT1-GFP (hygromycin resistance).
c
Transmission efficiency (TE) was calculated as #R/#S. dχ2 was calculated based on the expectation of a 1:1 segregation of transgenes in the resulting F1 populations. eThe aggregate numbers of F1 progeny from at least three independent complemented lines are represented.