Figure 11.

Interactions and synergistic actions of BRI1 (a BR receptor) and AHA2 (a PM H⁺-ATPase) under VHES and OHES. A, BRI1 and AHA2 were located in the PM. TM, Transmembrane. B, Split-ubiquitin Y2H assay for the interaction between BRI1 and AHA2. DDO, Double Dropouts; X, X-α-Gal; A, Aureobasidin A. C and D, BiFC assays showing in vivo interactions of BRI1 with AHA2 in tobacco (Nicotiana tabacum) leaf epidermal cells (C) and Arabidopsis protoplasts (D). All the images were taken by confocal microscopy. DIC, Differential interference contrast. Bars = 20 μm (C) and 10 μm (D). E and F, Gene relative expression of AHA2 or BRI1 in Arabidopsis root tips (5 mm from the root cap junction) of Ws and C24 under VHES (E) and OHES (F). Relative gene expression of AHA2 or BRI1 in C24 under VHES or OHES, respectively, is considered as 1-fold. The values shown are means, and error bars represent sd of six replicates from two independent experiments. Changes in the relative expression levels of a gene were checked for statistical significance according to Student’s t test (P < 0.05).