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. 2018 Feb 27;176(4):2590–2600. doi: 10.1104/pp.17.01776

Table II. Selected probes to investigate the dynamics of different plant wall polysaccharides.

Additional antibodies with known targets that are not listed here are available from PlantProbes (http://plantprobes.net) and CarboSource (https://www.ccrc.uga.edu/∼carbosource/CSS_home.html). CBMs, Carbohydrate-binding modules; Kdo, 3-deoxy-d-manno-oct-2-ulosonic acid; mAb, monoclonal antibody.

Polysaccharide Probe Target Reference
Cellulose Calcofluor White Various β-glucan structures Anderson et al. (2010)
Pontamine Scarlet 4B (S4B) Specific for crystalline cellulose Anderson et al. (2010)
FP-tagged CESA proteins Proxy for cellulose synthesis Paredez et al. (2006)
CBM3a Crystalline cellulose Blake et al. (2006)
CBM28 Amorphous cellulose Blake et al. (2006)
Pectin Ruthenium Red (RR) Pectin with deesterified GalA Hanke and Northcote (1975)
LM19 mAb HG, particularly deesterified forms Verhertbruggen et al. (2009)
2F4 mAb Unesterified HG cross-linked by Ca2+ Liners and Van Cutsem (1992)
LM20 mAb Methylesterified HG Verhertbruggen et al. (2009)
JIM7 mAb Methylesterified epitopes of HG Knox et al. (1990)
CCRC-M36 mAb RG I backbone Ruprecht et al. (2017)
Chitosan oligosaccharides (COS) Deesterified GalA regions Mravec et al. (2014)
Oligogalacturonides Unesterified HG cross-linked by Ca2+ Mravec et al. (2017)
Propidium iodide (PI) Unesterified GalA units Rounds et al. (2011)
Alkyne derivative of Fuc RG I Anderson et al. (2012)
Azido derivative of Kdo RG II Dumont et al. (2016)
Xyloglucan Sulforhodamine labeling Xyloglucan oligosaccharides Vissenberg et al. (2005)
CCRC-M1 mAb FucogalatoXyG Puhlmann et al. (1994)
Mannan LM21 mAb Branched and unbrached mannans Marcus et al. (2010))
LM22 mAb Unbranched mannans Marcus et al. (2010)
Xylan CBM15-mOrange2 (OC15) Xylan (xylohexaose) Khatri et al. (2016)
LM10 mAb Unbranched xylan McCartney et al. (2005)
LM11 mAb Branched xylan (e.g. with arabinose) McCartney et al. (2005)
LM28 mAb Glucuronosyl-substituted xylans Cornuault et al. (2015)