Table II. Selected probes to investigate the dynamics of different plant wall polysaccharides.
Additional antibodies with known targets that are not listed here are available from PlantProbes (http://plantprobes.net) and CarboSource (https://www.ccrc.uga.edu/∼carbosource/CSS_home.html). CBMs, Carbohydrate-binding modules; Kdo, 3-deoxy-d-manno-oct-2-ulosonic acid; mAb, monoclonal antibody.
Polysaccharide | Probe | Target | Reference |
---|---|---|---|
Cellulose | Calcofluor White | Various β-glucan structures | Anderson et al. (2010) |
Pontamine Scarlet 4B (S4B) | Specific for crystalline cellulose | Anderson et al. (2010) | |
FP-tagged CESA proteins | Proxy for cellulose synthesis | Paredez et al. (2006) | |
CBM3a | Crystalline cellulose | Blake et al. (2006) | |
CBM28 | Amorphous cellulose | Blake et al. (2006) | |
Pectin | Ruthenium Red (RR) | Pectin with deesterified GalA | Hanke and Northcote (1975) |
LM19 mAb | HG, particularly deesterified forms | Verhertbruggen et al. (2009) | |
2F4 mAb | Unesterified HG cross-linked by Ca2+ | Liners and Van Cutsem (1992) | |
LM20 mAb | Methylesterified HG | Verhertbruggen et al. (2009) | |
JIM7 mAb | Methylesterified epitopes of HG | Knox et al. (1990) | |
CCRC-M36 mAb | RG I backbone | Ruprecht et al. (2017) | |
Chitosan oligosaccharides (COS) | Deesterified GalA regions | Mravec et al. (2014) | |
Oligogalacturonides | Unesterified HG cross-linked by Ca2+ | Mravec et al. (2017) | |
Propidium iodide (PI) | Unesterified GalA units | Rounds et al. (2011) | |
Alkyne derivative of Fuc | RG I | Anderson et al. (2012) | |
Azido derivative of Kdo | RG II | Dumont et al. (2016) | |
Xyloglucan | Sulforhodamine labeling | Xyloglucan oligosaccharides | Vissenberg et al. (2005) |
CCRC-M1 mAb | FucogalatoXyG | Puhlmann et al. (1994) | |
Mannan | LM21 mAb | Branched and unbrached mannans | Marcus et al. (2010)) |
LM22 mAb | Unbranched mannans | Marcus et al. (2010) | |
Xylan | CBM15-mOrange2 (OC15) | Xylan (xylohexaose) | Khatri et al. (2016) |
LM10 mAb | Unbranched xylan | McCartney et al. (2005) | |
LM11 mAb | Branched xylan (e.g. with arabinose) | McCartney et al. (2005) | |
LM28 mAb | Glucuronosyl-substituted xylans | Cornuault et al. (2015) |