Figure 10.

Transcriptome effects induced by CsMADS6 overexpression in transgenic tomato sepals. A, Heat map of selected carotenoid-associated DEGs from the M6SvsWTS comparison. The column represents the comparison of M6S (CsMADS6-overexpressing sepals) with WTS (wild-type sepals), and each row represents an individual gene. The intensity of log₂ (fold change) is visualized using colors: red, up-regulated; blue, down-regulated. Gene identifiers and names are indicated at the left of each row. B, Schematic of the global transcriptional regulation by CsMADS6. Solid or dashed arrows indicate direct or indirect reaction flows in the pathway, respectively. The carotenoid-associated DEGs encoding enzymes that catalyze the reaction are shown at the sides of the arrows. Red and blue text denote expression levels with significant increases and decreases (P < 0.05), respectively. Yellow boxes highlight the critical intermediates in the metabolic pathways. The thick red and blue arrows indicate up- and down-regulation of the metabolic flux, respectively. Other processes, including primary pathways, secondary pathways, and phytohormone and TF levels (highlighted with yellow ovals), and their relation to accumulation of large amounts of a specific carotenoid (lycopene) in the transgenic sepals, are indicated. ABA, Abscisic acid; SLs, strigolactones.