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. 2018 Feb 20;176(4):2657–2676. doi: 10.1104/pp.17.01830

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Figure 7. — Effects of CsMADS6 overexpression in tomato fruits. A, Phenotypes of transgenic tomato fruits. WT, The wild type; Ox-61, an independent CsMADS6-overexpressing line; MG, mature green; Br+10, 10 d after breaker stage. Note the succulent sepals in the transgenic lines. Bar = 1 cm. B, RT-PCR and immunoblotting analysis of CsMADS6 expression. Act, ACTIN gene (internal reference). C and D, Carotenoid content in transgenic tomato pericarps (C) and sepals (D) at the Br+5 stage of fruit development. Lut, Lutein; β-Car, β-carotene; Lyc, lycopene; Total, total carotenoids; DW, dry weight. The data are expressed as means ± sd from three biological replicates. Statistically significant differences are indicated with either single asterisks or lowercase letters (P < 0.05) and double asterisks or uppercase letters (P < 0.01) by one-way ANOVA tests. Different letters within each column indicate significant differences.