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. 2018 Feb 12;176(4):2991–3002. doi: 10.1104/pp.17.01757

Figure 2.

Figure 2.

The kinase domain of MAPKKK5 is essential for the interaction with BSK1. A, The different domains ofMAPKKK5 are shown. MAPKKK5 contains a kinase domain in the middle, in addition to N-terminal and C-terminal domains. The numbers indicate the positions of amino acid sequences. B, The kinase domain of MAPKKK5 interacted with BSK1 in the yeast two-hybrid assay. Corresponding constructs were cotransformed into yeast strain AH109. A 10 μL suspension of each pair was dropped on the indicated SD medium. C, The kinase domain of MAPKKK5 interacted with BSK1 in the BiFC assay. Leaves of N. benthamiana were infiltrated with A. tumefaciens strain GV3101 containing the indicated construct pairs. BSK1 and truncated MAPKKK5 proteins were fused to the YN or YC. YFP signal was detected by confocal microscopy. Bar = 50 μm. AD, pGADT7; BD, pGBKT7; CT, C-terminal domain; KD, kinase domain; KD:C, MAPKKK5 lacking the N terminus of 345 amino acids; N:KD, MAPKKK5 lacking the C terminus of 109 amino acids; NT, N-terminal domain.