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. 2018 Feb 12;176(4):2991–3002. doi: 10.1104/pp.17.01757

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Figure 6. — MAPKKK5-induced cell death is independent of Ser-289 phosphorylation. Different MAPKKK5 variants were transiently expressed in N. benthamiana. The infiltrated leaves were photographed (left) and then stained with Trypan Blue (right) for observation of dead cells at 3 dpi. Similar cell death was induced in the leaves expressing MAPKKK5-YFP-HA, MAPKKK5^S289A-YFP-HA, or MAPKKK5^S289D-YFP-HA, but not in the leaves expressingMAPKKK5^K375M-YFP-HA. 1, MAPKKK5-YFP-HA; 2, MAPKKK5^K375M-YFP-HA; 3, MAPKKK5^S289A-YFP-HA; 4, MAPKKK5^S289D-YFP-HA.