Figure 12.
Effects of the constitutive expression of HsfA2 on the growth and heat tolerance of rabe1b-1. A, Constitutive expression of the HsfA2 transgene in transgenic rabe1b plants. Total RNA was isolated from Arabidopsis Col-0 wild type, the rabe1b mutant, and two independent lines of transgenic rabe1b mutant plants harboring the HsfA2 transgene under the control of the CaMV 35S promoter. HsfA2 transcript levels were determined using RT-qPCR. Means and se were calculated from three replicates. B, Fv/Fm images of fully expanded leaves determined immediately after 0 and 4 h at 45°C. The color code in the images ranged from 0 (black) to 1 (purple). C, Fv/Fm values of fully expanded leaves determined immediately after the indicated hours of heat treatment at 45°C. According to Duncan’s multiple range test (P < 0.01), means of Fv/Fm at 0 h do not differ significantly if they are indicated with the same lowercase letter; means of Fv/Fm at 4 h do not differ significantly if they are indicated with the same uppercase letter.