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Effects of the constitutive expression of HsfA2 on HsfA2 and HsfA2 target genes. Five-week-old Arabidopsis wild-type Col-0, the rabe1b-1 mutant, and the rabe1b mutant constitutively expressing HsfA2 were treated by heat stress at 45°C for 0 and 1 h. Total RNA was isolated from leaf samples, and transcript levels of the indicated genes were determined using RT-qPCR. Means and se were calculated from three replicates.
