Figure 6.
Effects of overexpression of Rabe1b, Rabe1b-GTP, and Rabe1b-GDP on the heat tolerance of Arabidopsis mature plants. A, Six-week-old mature plants were placed in a 45°C growth chamber for 10 h. The heat-treated plants were then moved to a 23°C growth chamber for recovery. The photograph was taken 5 d after the heat treatment. B, Fv/Fm images of fully expanded leaves determined immediately after the indicated hours (h) at 45°C heat treatment. The color code in the images ranged from 0 (black) to 1 (purple). C, Fv/Fm values of fully expanded leaves determined immediately after the indicated hours of heat treatment at 45°C. Means and se were calculated from average Fv/Fm values determined from three experiments with 10 leaves per experiment for each genotype. According to Duncan’s multiple range test (P < 0.01), means of Fv/Fm at 0 h do not differ significantly if they are indicated with the same lowercase letter; means of Fv/Fm at 6 h do not differ significantly if they are indicated with the same uppercase letter.