Figure 3.

Functional characterization of ZmCYP71Z16. A, Maximum likelihood phylogenetic tree of select P450 proteins of the CYP71 family (Supplemental Table S2). The tree is rooted with an uncharacterized CYP71 of Amborella trichopoda. Circles indicate bootstrap support of greater than 80% (500 repetitions). Demonstrated activities in diterpenoid (red), monoterpenoid/sesquiterpenoid (blue), and other specialized metabolic pathways (purple) are highlighted. B, Total ion chromatograms (TIC) and mass spectra of products 1, 13, and 14 derived from microbial coexpression of ZmAN2, ZmKSL4, ZmCYP71Z16, and ZmCPR2. C, Structures of epoxydolabrene 13 and epoxydolabranol 14 as verified by NMR analysis.