Figure 4.

Occurrence of dolabralexins in planta. A and B, Average quantities of dolabradiene and epoxydolabranol (A) and trihydroxydolabrene (B) in field-grown roots of B73, Mo17, and hybrid sweet corn (Golden Queen [GQ]). Error bars represent propagated se values across four biological replicates. Letters represent significant differences at P < 0.05 as measured using ANOVA and Tukey’s tests to correct for multiple comparisons between control and treatments. FW, Fresh weight. C, Analysis of trihydroxydolabrene extracted from roots using LC-MS/MS analysis, with traces representing combined extracted ion chromatograms (m/z 305–305.5 + 645.25–645.75). In-source decay of the [2M+H]⁺ m/z 645.5 trihydroxydolabrene parent ion yields [M-H₂O+H]⁺ m/z 305.25, occurring at a retention time of 27.6 min. MS/MS analyses of the trihydroxydolabrene-derived m/z 305.25 results in highly similar fragmentation patterns as compared with the enzyme-produced epoxydolabranol analytical standard (retention time = 36.7 min).