Fig 4. Loss of local astrocyte support does not affect basal or high-demand transmission following prolonged glucose deprivation.

(A,B) Representative baseline evoked EPSCs (black traces) and subsequent recovery following K⁺-evoked vesicle depletion (gray traces) of -astrocyte neurons incubated in conditioned medium (A, Control) and following prolonged glucose deprivation (B, 0 Glucose). (C) Summary of baseline charge transfer following prolonged incubation in either conditioned medium (Control; black bar/symbols) or glucose deprivation (red bar/symbols; p > 0.05, Student’s t-test). (D) Summary of recovery of evoked EPSCs following K⁺-induced vesicle depletion for conditioned medium controls (black trace) compared to glucose deprivation (red trace; p > 0.05; Two-way repeated measures ANOVA). Data are represented as mean ± SEM. n.s., non-significant.