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. 2018 Mar 19;14(3):e1006882. doi: 10.1371/journal.ppat.1006882

Fig 5. Localization of EHI_175690, and protrusion formation on EHI_175690-HA-expressing amoebae.

Fig 5

(A) Immunofluorescence image, obtained by confocal microscopy, showing localization of EHI_176590. EHI_176590-HA-expressing amoebae were fixed and reacted with anti-HA antibody. (B) Magnified image of the inset in (A). Arrows indicate protrusions. (C) Immunofluorescence image, obtained by confocal microscopy, showing localization of EHI_176590-HA and the intermediate subunit of galactose/N-acetylgalactosamine-specific lectin (Igl). EHI_176590-HA-expressing amoebae were fixed and stained with anti-HA and anti-Igl antibodies. Arrow indicates protrusion. Islet showing signal-enhanced image to highlight the protrusion. (D) Effect of EHI_176590-HA expression on the percentage of amoebae that possess protrusions. EHI_176590-HA-expressing and mock transformants (HA) were immunostained as in (C) and the number of cells with protrusions, longer than 0.7 μm, was counted and are presented as percentages. The averages of three independent experiments, in which 100–200 amoebae were counted, are shown. (E) Effect of EHI_176590-HA expression on the number of protrusions per amoeba. EHI_176590-HA-expressing and mock transformants (HA) were immunostained as in (C), and the number of protrusions, per amoeba, was counted. The averages of three independent experiments, in which 100–200 amoebae were counted, are shown. * indicates p<0.01.