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. 2018 Mar 19;14(3):e1006882. doi: 10.1371/journal.ppat.1006882

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© 2018 Nakada-Tsukui et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — EHI_176590-HA expression in KU27 (A) Immuno-detection of EHI_176590-HA ectopically expressed in strain KU27. Total cell lysates, from mock-transfected and EHI_176590-HA-expressing strain KU27 trophozoites, were analyzed by immunoblot analysis with anti-EHI_176590 (left panel) and anti-HA antibody (right panel). The arrow indicates EHI_176590-HA, and asterisks indicate protein bands detected by non-specific immunoreactivity. (B) Low-magnification confocal immunofluorescence images of EHI_176590-HA-expressing and mock-transfected KU27. Trophozoites were fixed and reacted with anti-Igl and anti-HA antibodies. Merged fluorescent (IGL/HA) and DIC images are also shown. (C) Adhesion of the parental, EHI_176590-HA-expressing, and mock-transfected KU27 to HRBCs. Parental (open bars), EHI_176590-HA-expressing (black bars), and mock-transfected (gray bars) KU27 strains were co-cultured with HRBCs on ice for 30 min, and adherent HRBCs per ameba were counted. The total number of trophozoites was set to 100%, and the percentage of trophozoites, bound to 0–5, 6–10, or >10 HRBCs, are shown. Error bars indicate standard deviations of four biological replicates.