Fig. 1.

3D clustering of co-regulated genes. a Two copies of GAL1 genes (red) at allelic locations in diploid yeast cluster together in a transcriptional hotspot (yellow circle) upon galactose induction. b A fraction of Met4-targeted genes (red) form 3D cluster in a transcriptional hotspot. Double arrows indicate the physical interactions between different genes in the cluster. c Three NFκB-regulated genes (Gene 1, 2, and 3 shown in blue, green, and red, respectively) form a multigene complex with hierarchy: transcription of Gene 1 is required for the recruitment and expression of Gene 2, and is then followed by the recruitment and expression of Gene 3. Gray circles represent the transcription machineries