Fig.6. Wild-type, mutant, and null E. coli growth signals at OD₅₉₅ in the presence and absence of safranal and damascenone.

200 µl E. coli culture was grown in limiting glucose and succinate with maximal inhibitory concentrations of safranal (10 mM) and damascenone (8 mM) in 96-well plates. Optical density at λ595 nM was set to be measured every 10 min for 25 hours with shaking. Experimental details are given in the Materials and Methods section. Each data point is the average of at least three sample readings.