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. 2018 Feb 26;9(21):15606–15615. doi: 10.18632/oncotarget.24581

Figure 2. Osmotic lysis of cancerous breast cells.

Figure 2

(A) The photomicrographs were taken from best response video recordings that depict interval changes in cellular integrity of MDA-MB-231 cells in culture that were treated with ouabain (100 nM) and then stimulated with 1.0 V DC delivered in 1 msec pulse trains at 200 pps at stimulus onset (time 0) and after 8 sec and 16 sec of continuous stimulation. Note the bulging of cell membranes and the exudation of cytosol (arrows) into the surrounding media that is indicative of cell lysis. (B) The histogram depicts the average time to lysis of MCF-10a, MCF-7 and MDA-MB-231 cells treated with TOL. The results are consistent with the hypothesis that the rate of TOL is dependent on the relative number of sodium channels expressed in each of the cell types. Note that TOL spares the cells derived from normal breast tissue (MCF-10a). *p < 0.05; **p < 0.01.