Figure 7. PLOD3 regulates p21 and hypoxia induced HIF-1α expression in GBM cells.
Expression of HIF-1α, p53, p21, EMT markers (E-cadherin, N-cadherin, Snail, and Twist), and PI3K/AKT/mTOR signaling pathway proteins (p-AKT (Ser473), total AKT, p-ERK (Thr202/Tyr204), total ERK, p-mTOR (Ser2448), and total mTOR) was analyzed by western blotting in shLuc, shPLOD3 GBM8401, and LN229 cells under normoxic and hypoxic conditions. The data are representative of two independent experiments. β-actin served as a loading control.