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. 2018 Feb 28;9(21):15721–15731. doi: 10.18632/oncotarget.24595

Figure 4. IL-8 is upregulated in MES GSCs and mediates in vitro angiogenesis.

Figure 4

Expression levels of ANGPT2, VEGFA, and IL8 mRNA are upregulated in MES versus PN GBMs (TCGA cohort, A–C). MES GSCs (GSC 20, 28 and 267) display higher levels of the mRNA transcript in comparison to PN GSCs (GSC 8-11, 11 and 23) (D). The secretion level of IL-8 also indicated increased IL-8 secretion by MES GSCs in comparison to PN GSCs (E). CM of GSCs only had minor effects on HMEC-1 proliferation, with a trend for inhibition of HMEC-1 proliferation when an IL-8 was neutralized in MES GSC28 CM (F). Representative images of tube formation assays are displayed (G) that illustrate enhanced tube formation by the addition of MES CM and significant inhibition of tube formation when IL-8 is neutralized in GSC28 CM (H). The variation in individual assays is also shown (I, J). Assays were repeated five (proliferation) or six (tube formation) times and mean values with standard error of the mean (SEM) are plotted; *P < 0.05, ***P < 0.001.