Table 2.

Genes found in the screen

Gene name	Screen, criteria	Important for	Description
ARG82	U,2	AA metabolism	Inositol polyphosphate multikinase
ERV46	U,1,4,5	Cargo transport	ER vesicle protein, component of COPII complex; required for membrane fusion
HIS1	U,1,4,5	AA metabolism	ATP phosphoribosyltransferase
UGA1	U,5	AA metabolism	Gamma‐aminobutyrate (GABA) transaminase
SLA1	U,2,3	Actin binding	Cytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton
SAP155	K,2,3,5	Cell cycle	Protein required for function of the Sit4 protein phosphatase
YER068C‐A	U,5	Dubious open reading frame	Dubious open reading frame/overlaps with ARG5, ARG6 acetylglutamate kinase and N‐acetyl‐gamma‐glutamyl‐phosphate reductase
YIL032C	U,5	Dubious open reading frame	Dubious open reading frame/next to BCY1
ERG3	U,1,5	Ergosterol biosynthesis	C‐5 sterol desaturase
GAL83	K,1	Glucose repression	One of three possible beta‐subunits of the Snf1 kinase complex
GUP1	U,2,3	Glycerol metabolism, protein folding	Plasma membrane protein involved in remodeling GPI anchors
PPZ1	K,5	Ion homeostasis	Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
FUS1	U,5	Mating	Membrane protein localized to the shmoo tip
AAT2	U,5	Metabolism	Cytosolic aspartate aminotransferase involved in nitrogen metabolism
PTC6	K,2,3	Metabolism	Mitochondrial type 2C protein phosphatase (PP2C)
GIM4	U,5	Microtubule chaperone/Protein folding	Subunit of the heterohexameric cochaperone prefoldin complex
PAC10 GIM2	U,5	Microtubule chaperone/Protein folding	Subunit of the heterohexameric cochaperone prefoldin complex
BIM1	U,4,5	Microtubule end binding	Microtubule plus‐end‐binding protein
MSH1	U,5	Mitochondrial homeostasis	Escherichia coli MutS homolog, binds DNA mismatches, required for mitochondrial function
BUB1	K,4,5	Mitosis	Protein kinase required for cell cycle checkpoint, delays entry into anaphase until kinetochores bound by opposing microtubules
ELM1	K,2	Morphogenesis	Serine/threonine protein kinase that regulates cellular morphogenesis
HSL1	K,2	Morphogenesis	Nim1‐related protein kinase; regulates the morphogenesis and septin checkpoints
NUP60	U,5	Nuclear transport	FG‐nucleoporin component of central core of the nuclear pore complex
SXM1	U,4,5	Nuclear transport	Nuclear transport factor (karyopherin)
CBR1	U,5	Respiration	Microsomal cytochrome β reductase
RTC3	U,4,5	RNA metabolism	Protein of unknown function involved in RNA metabolism
CKA1	U,1,4,5	Signaling	Alpha catalytic subunit of casein kinase 2 (CK2)
CKB1	U,5	Signaling	Beta regulatory subunit of casein kinase 2 (CK2)
CKB2	K,1	Signaling	Beta' regulatory subunit of casein kinase 2 (CK2)
FUS3	K,3,5	Signaling	Mitogen‐activated serine/threonine protein kinase (MAPK), part of PRS
HOG1	K,3	Signaling	Mitogen‐activated protein kinase involved in High Osmolarity (HOG) pathway
KSS1	K,3	Signaling	Mitogen‐activated protein kinase (MAPK); functions in PRS and signal transduction pathways that control filamentous growth and pheromone response
PBS2	K,5	Signaling	MAP kinase kinase of the HOG signaling pathway
SSK2	K,3	Signaling	MAP kinase kinase kinase of HOG1 signaling pathway
FAR1	U,1	Signaling/cell cycle/polarization	CDK inhibitor, nuclear anchor, recruited by Ste18‐Ste4 at polarity patch
SIP1	U,4,5	Signaling/glucose repression	Alternate beta‐subunit of the Snf1 protein kinase complex
KAR4	U,5	Signaling/mating	Transcription factor required for activation of some pheromone‐responsive genes
STE50	U,1,4	Signaling/mating	Adaptor protein, in PRS helps connect Ste20 MAPKKKK to Ste11 MAPKKK
SKY1	K,3	Splicing	SR protein kinase (SRPK); varied functions, regulates proteins involved in mRNA metabolism and cation homeostasis, helps some LexA fusion proteins bind operator
KIN3	K,5	Stress	Non‐essential serine/threonine protein kinase; possible role in DNA damage response
OCA1	K,1	Stress	Protein tyrosine phosphatase; required for cell cycle arrest in response to oxidative damage of DNA
CTK1	K,4	Transcription regulation	Catalytic (alpha) subunit of C‐terminal domain kinase I (CTDK‐I); phosphorylates RNA pol II
DEP1	U,5	Transcription regulation	Component of the Rpd3L histone deacetylase complex, variously needed for activation and repression, regulates DNA replication origin timing
SUM1	U,2,4,5	Transcription regulation	Transcriptional repressor that regulates middle‐sporulation genes; required for mitotic repression of middle‐sporulation‐specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing
SWI5	U,5	Transcription regulation	Part of Mediator and Swi/Snf nucleosome remodeling complexes
UME6	U,4,5	Transcription regulation	Meiotic transcription regulator, DNA binding, recruits variously Sin3/Rpd3 repressor (HDAC) and Ime1 activator.
RPL12A	U,1,4	Translation	Ribosomal 60S subunit protein L12A
RPL19B	U,1,4,5	Translation	Ribosomal 60S subunit protein L19B
RPL34A	U,4,5	Translation	Ribosomal 60S subunit protein L34A
ECM15	U,1,4,5	Unknown	Possibly tetrameric, non‐essential protein, unknown function
VPS64	U,5	Vacuole metabolism	Required for cytoplasmic proteins to enter vacuole

Selection criteria codes (see Fig 2A–C): (1) O(0.6 nM) < 3.39; (2) O(0.6 nM) > 7.72; (3) η²(P(0.6 nM)) < 0.027; (4) η²(P(0.6 nM)) > 0.054; (5) η²(P(20 nM)) > 0.019. Genes from the strains in the unbiased (U) screen and the strains in the non‐essential kinase and phosphatase screen (K) screen that showed altered PRS system output (O), low or high pathway variability (η²(P)) at low pheromone (0.6 nM), or high variability (η²(P)) at high dose (20 nM). Table shows gene name, screen from which it was selected and selection criteria, overall functional class, and a brief description of its molecular role or activity.