Fig. 2. Room temperature neutron structure of AAC-VIa.

Close-up view of the neutron diffraction model and nuclear density for sisomicin-bound AAC-VIa. Sisomicin-bound active-site residues are colored with magenta carbon atoms, and sisomicin with green carbon atoms. His¹⁸⁹ from the neutron diffraction data acquired with the apo-enzyme is shown and colored with tan carbon atoms. The 2Fo-Fc nuclear density map is shown in light gray. The Fo-Fc deuterium nuclear omit map for the proton involved in the LBHB is shown in green. Hydrogen bonds are represented as red dashed lines.