Table 2. Binding specificity and haptenic sugars of lectins for profiling of tissue glycosylation.
A haptenic sugar is a monosaccharide or disaccharide that can inhibit binding of a lectin.
Lectin | Binding specificity | Haptenic sugar (100 mM) |
SNA-I | Sialic acid-α-(2,6)–Gal(NAc)–R | Lactose |
MAA | Sialic acid-α-(2,3)–Gal(NAc)–R | Lactose |
WGA | Sialic acid, GlcNAc | GlcNAc |
PNA | Gal-β-(1,3)-GalNAc (T antigen), > GalNAc > lactose > Gal, terminal β-Gal, nonsialylated | Gal |
GS-I-B4 | Terminal α-linked Gal | Gal |
Con A | α-Linked Man, GalNAc and GlcNAc | Man |
UEA-I | α-(1,2)–Linked fucose | Fucose |
WFA | GalNAc and CS | GalNAc |