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Isoproterenol (Iso) increased the spontaneous action potential (AP) and Ca2+ transient firings (chronotropy) of single hES2-ventricular microtissues (vCMs) but not the transient amplitude. A, Representative AP tracings of single hES2-vCMs before and after Iso. B, The corresponding AP firing frequencies (n=4; **P=0.008). C, Representative recordings of spontaneous Ca2+ transients. D, Transient rate (**P=0.005) and amplitude (P=0.182) of Ca2+ transients (n=6).
