Table 1.
Gene | Forward primer | Reverse primer |
---|---|---|
hPCNA | 5′- GTCTCTTTGGTGCAGCTCA -3′ | 5′- ATCTTCGGCCCTTAGTGTAATG -3′ |
hFABP4 | 5′- CATGTGCAGAAATGGGATGG -3′ | 5′- AACTTCAGTCCAGGTCAACG -3′ |
hPDK4 | 5′- TGAGACTCGCCAACATTCTG -3′ | 5′- TCTGGTCATCTGGGCTTTTC -3′ |
hPLIN2 | 5′- GCTCCATTCTACTGTTCACCTG -3′ | 5′- CTCCTTTTCCACTCTACCCATG -3′ |
hMUC1 | 5′- TCCTCACAGTGCTTACAGTTG -3′ | 5′- AGAAAGAGACCCCAGTAGACA -3′ |
hCK8 | 5′- AAGGCACAGTACGAGGATATTG -3′ | 5′- AGATCTCAGTCTTTGTGCGC -3′ |
hESR1 | 5′- ATCTCGGTTCCGCATGATGAATCTGC -3′ | 5′- TGCTGGACAGAAATGTGTACACTCCAGA -3′ |
hPgR | 5′- ATTACCAGTGTTCCCGTCTTC -3′ | 5′- GTGCTCCAATAGTTCCCTGTAC -3′ |
hCK6a | 5′- TCCCCTCAACCTGCAAATC -3′ | 5′- CCACTTTGTTTCCAGAACCTTG -3′ |
hCK6b | 5′- AGCTGAGAAACATGCAGGAC -3′ | 5′- GCTTGCAGTTCAACCTTGTTC -3′ |
hCK17 | 5′- AACAAGATCCTCACAGCCAC -3′ | 5′- CCATTGATGTCGGCCTCC -3′ |
hGAPDH | 5′- CCCACATGGCCTCCAAGGAGTA -3′ | 5′- GTCTACATGGCAACTGTGAGGAGG -3′ |
hβ2m | 5′- CTTAGCTGTGCTCGCGCTACTCT -3′ | 5′- CCATTCTCTGCTGGATGACGTGAGT -3′ |
mFABP4 | 5′- GACAGGAAGGTGAAGAGCATC -3′ | 5′- GTCACGCCTTTCATAACACATTC -3′ |
mPDK4 | 5′- AGTGACTCAAAGACGGGAAAC -3′ | 5′- GTGTGAGGTTTAATTCTGGCG -3′ |
mPLIN2 | 5′- GGATAAGCTCTATGTCTCGTGG -3′ | 5′- GTCTGGCATGTAGTCTGGAG -3′ |
mCK8 | 5′- TGTTGAGCCCCTTGAAGC -3′ | 5′- GGTCTCCAGCATCTTGTTCTG -3′ |
m CK18 | 5′- ACACCAACATCACAAGGCTG -3′ | 5′- TTCCACAGTCAATCCAGAGC -3′ |
mESR1 | 5′- AACCGCCCATGATCTATTCTG -3′ | 5′- AGATTCAAGTCCCCAAAGCC -3′ |
mPgR | 5′- TGAGCCTGATGGTGTTTGG -3′ | 5′- ACAGCGAGTAGAATGACAGC -3′ |
mACTIN | 5′- ATTTCTGAATGGCCCAGGTC -3′ | 5′- GCTGCCTCAACACCTCAA -3′ |
MUC1 mucin 1, CK cytokeratin, ESR1 estrogen receptor 1, PgR progesterone receptor, β2m β2-microglobulin