Table 2.
Antibodies used for immunohistochemistry and western blot analyses
| Antibody | Source | References | Clone | Species | Dilution |
|---|---|---|---|---|---|
| CD44 | ThermoFisher Scientific Inc. | MA1-39422 | SPM521 | Mouse | 1:75 |
| P63 | Santa Cruz Biotechnology | sc-8431 | 4A4 | Mouse | 1:400 |
| Cytokeratin 8 | Abcam | ab59400 | Rabbit | 1:100 | |
| PCNA | Santa Cruz Biotechnology | sc-7907 | FL-261 | Rabbit | 1:300 |
| Phospho-Akt (Ser473) | Cell Signaling | 4051 | 587F11 | Mouse | 1:1000 |
| Total Akt | Cell Signaling | 9272 | Rabbit | 1:1000 | |
| Phospho-ERK1/2 (Thr202/Tyr204) | Cell Signaling | 9101 | Rabbit | 1:1000 | |
| Total ERK1/2 | Cell Signaling | 9102 | Rabbit | 1:1000 | |
| Actin | Millipore | MAB1501 | C4 | Mouse | 1:3000 |