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. 2018 Apr 4;8:5618. doi: 10.1038/s41598-018-23918-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lmna^−/− myoblasts premature differentiation. (A) Phase-contrast photographs and immunofluorescence labelling of MyHC (in green) and DAPI (in blue) of wild-type and Lmna^−/− H-2K MB in proliferation and after 48 hours of differentiation. Scale bar: 400 µm for proliferation and 200 µm for differentiation. (B) Quantification of the differentiation index (percentage of nuclei in MyHC positive cells) in wild-type and Lmna^−/− H-2K myotubes after 48 hours of differentiation. (C,D) Expression of Myogenin measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) (C) and western blot (D) in wild-type and Lmna^−/− proliferating myoblasts (P, Proliferation) and during muscle differentiation (from 6 hours to 48 hours of differentiation). (E,F) Expression of Id1 (E) and Id2 (F) measured by qRT-PCR in wild-type and Lmna^−/− proliferating myoblasts (P, Proliferation) and during muscle differentiation (from 6 hours to 48 hours of differentiation). Expression is shown as mean (± SEM) of biological replicates (n = 4). Values are relative expression normalized on Hprt1 housekeeping gene expression. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Full-length blots are presented in Supplementary Figure 4.