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. 2018 Apr 4;8:5663. doi: 10.1038/s41598-018-23939-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Endogenous MMP deactivation and microtensile strength after CuBGn@DA application to dentin. (A) Fluorescent images reveal the successful application of CuBGn (blue)-incorporated DA (green) on dentin (mineralized matrix, red). Auto-fluorescence of composite resin was also shown as blue (B) Endogenous MMP activity was significantly decreased in CuBGn-DA compared to that in the controls (BGn-DA, DA, and acetone (solvent of DA)) after application of DA to dentin etched with 37% phosphoric acid. (C) Microtensile strength was not compromised after the incorporation of nanoparticles (n = 20). Different letters indicate significant differences between groups at a level of 0.05.