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. 2018 Apr 4;8:5632. doi: 10.1038/s41598-018-24014-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Endothelial deletion of rudhira leads to unpatterned extraembryonic vasculature. (a) Yolk sac vasculature marked by PECAM staining in control and rudh^CKO (rudhfl/fl;TekCre+) at E10.5 and E11.5. Red dot: primary blood vessel; green dot: secondary blood vessel; yellow dot: tertiary blood vessel. Graphs show quantitation of number of secondary vessels, branch points and lumen size in control and rudh^CKO at E10.5 and E11.5. (b–e) Placental morphology seen by histological analysis (b, d) or immunofluorescence staining (c,e) in sections of control and rudh^CKO as indicated. Boxed areas show lower magnification of placentas (b,d). Immunostaining shows fetal and maternal blood cell pockets marked by Ly76. Nuclei are marked by DAPI (Blue). Arrows: maternal blood cells; arrowheads: fetal blood cells. de: decidua, sp: spongiotrophoblast, lb: labyrinth, ch: chorion. Graph shows quantitation of placental thickness. Results shown are a representative of at least three independent experiments with at least three biological replicates. Statistical analysis was carried out using one-way ANOVA. Scale bar: (a) 200 μm; (b–e) 100 µm. *p < 0.05, **p < 0.01, ***p < 0.001.