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. 2018 Apr 4;8:5653. doi: 10.1038/s41598-018-23937-8

Figure 2.

Figure 2

Effect of transcription factors on Robo4 promoter activity in iPS-derived cells. (A) Expression of transcription factors in iPS-derived cells. Expression of SOX factors, VEZF1, and ETV2 in iPS-derived cells was measured by real-time RT-PCR. Data are means ± S.D. (n = 3). (B) Effects of transcription factors on Robo4 promoter activity. HEK293 cells were co-transfected with a Robo4 promoter-luciferase reporter construct and expression vectors for transcription factors or GFP, and luciferase activity was measured 24 h after transfection. Data are means ± S.D. (n = 3). *p < 0.05 by two-tailed Dunnett’s test vs pcDNA3. (C) Effect of transcription factors on Robo4 expression in ECs. Transcription factors or GFP were expressed from adenovirus vectors in HUVECs, and Robo4 expression was measured after 48 h by real-time RT-PCR (n = 3). *p < 0.05 by two-tailed Dunnett’s test vs GFP.