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. 2018 Apr 4;8:5653. doi: 10.1038/s41598-018-23937-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ETV2 binding to ETS motifs in the Robo4 promoter. (A) ETV2 binding to ETS motifs in Robo4 promoter. Electrophoretic mobility shift assay using ETV2 (E), control protein (C) and ³²P-labeled probes containing ETS motifs. A 50-fold molar excess of unlabeled wild type (WT) or mutant (Mut) ETS motif was used as competitor. The arrow indicates the shifted bands derived from an ETV2-DNA complex. (B) ETV2 binding to endogenous Robo4 promoter. Chromatin immunoprecipitation with anti-FLAG or control IgG of HUVECs expressing ETV2-FLAG or GFP. Immunoprecipitated DNA fragments were analyzed by real-time PCR targeting the Robo4 promoter. Data are means ± S.D. (n = 4). *p < 0.05 by two-tailed Student’s t-test vs control IgG.