Table 1.
Effect of the treatment with interferon-β, progesterone or cyclopamine of each HLA-G 5′URR constructions transfected into JEG-3 cell line.
| Haplotypes | Treatment | ||||||
|---|---|---|---|---|---|---|---|
| Interferon-β | Progesterone | Cyclopamine | |||||
| Delta | Ratio | Delta | Ratio | Delta | Ratio | 1-Ratio | |
| G0104a | 0.0244 | 2.0990 | 0.0136 | 1.2240 | −0.0056* | 0.7618 | 0.2382 |
| 0.0321 | 2.3500 | 0.0106 | 1.2330 | −0.0060 | 0.7514 | 0.2486 | |
| (0.0266) | (1.0770) | (0.0076) | (0.2010) | (0.0030) | (0.1186) | (0.1186) | |
| n = 8 | n = 8 | n = 8 | n = 8 | n = 10 | n = 10 | n = 10 | |
| G0104b | 0.0414 | 2.6980 | 0.0040 | 1.0880 | −0.0072 | 0.7462 | 0.2538 |
| 0.0379 | 2.4490 | 0.0024 | 1.0580 | −0.0079 | 0.7043 | 0.2957 | |
| (0.0239) | (0.8426) | (0.0086) | (0.2097) | (0.0037) | (0.1551) | (0.1551) | |
| n = 8 | n = 8 | n = 8 | n = 8 | n = 10 | n = 10 | n = 10 | |
| G010102a | 0.0240 | 2.0590 | 0.0082 | 1.1930 | −0.0074 | 0.6861 | 0.3139 |
| 0.0263 | 2.1520 | 0.0086 | 1.2140 | −0.0074 | 0.6626 | 0.3374 | |
| (0.0161) | (0.6401) | (0.0220) | (0.2048) | (0.0038) | (0.1862) | (0.1862) | |
| n = 8 | n = 8 | n = 8 | n = 8 | n = 10 | n = 10 | n = 10 | |
| G010101a | 0.0428 | 2.1930 | 0.0020 | 1.0080 | −0.0145* | 0.5833* | 0.4167* |
| 0.0507 | 2.1660 | 0.0337 | 1.2550 | −0.0176 | 0.6172 | 0.3828 | |
| (0.0361) | (0.7719) | (0.0595) | (0.5265) | (0.0055) | (0.1110) | (0.1110) | |
| n = 8 | n = 8 | n = 8 | n = 8 | n = 10 | n = 10 | n = 10 | |
| G010101b | 0.0604 | 3.5240 | 0.0057 | 1.1540 | −0.0065* | 0.7248 | 0.2752 |
| 0.0581 | 3.4360 | 0.0042 | 1.1120 | −0.0072 | 0.7330 | 0.2670 | |
| (0.0317) | (1.3240) | (0.0052) | (0.1234) | (0.0047) | (0.1247) | (0.1247) | |
| n = 8 | n = 8 | n = 8 | n = 8 | n = 10 | n = 10 | n = 10 | |
| G010101c | 0.0488 | 2.9900 | 0.0027 | 1.0650 | −0.0126# | 0.6854 | 0.3146 |
| 0.0474 | 3.5290 | 0.0086 | 1.1550 | −0.0116 | 0.6180 | 0.3820 | |
| (0.0307) | (2.1220) | (0.0156) | (0.2824) | (0.0049) | (0.1828) | (0.1828) | |
| n = 8 | n = 8 | n = 8 | n = 8 | n = 10 | n = 10 | n = 10 | |
| G010101d | 0.0252 | 2.8540 | 0.0160 | 1.1810 | −0.0032*# | 0.7867 | 0.2133 |
| 0.0278 | 2.9690 | 0.0181 | 1.1290 | −0.0035 | 0.8063 | 0.1937 | |
| (0.0160) | (0.8591) | (0.0194) | (0.1484) | (0.0020) | (0.0914) | (0.0914) | |
| n = 8 | n = 8 | n = 8 | n = 8 | n = 10 | n = 10 | n = 10 | |
| G010101f | 0.0291 | 2.2470 | 0.0218 | 1.2920 | −0.0119# | 0.6982 | 0.3018 |
| 0.0311 | 2.1530 | 0.0276 | 1.2940 | −0.0109 | 0.6870 | 0.3130 | |
| (0.0217) | (0.6191) | (0.0252) | (0.1730) | (0.0042) | (0.1137) | (0.1137) | |
| n = 8 | n = 8 | n = 8 | n = 8 | n = 10 | n = 10 | n = 10 | |
| G0103a | 0.0263 | 2.5260 | 0.0063 | 1.1710 | −0.0050* | 0.8310* | 0.1690* |
| 0.0267 | 2.3630 | 0.0130 | 1.4400 | −0.0040 | 0.8574 | 0.1426 | |
| (0.0170) | (0.7231) | (0.0174) | (0.6873) | (0.0041) | (0.1566) | (0.1566) | |
| n = 8 | n = 8 | n = 8 | n = 8 | n = 10 | n = 10 | n = 10 | |
| G0103e | 0.0283 | 2.4090 | 0.0070 | 1.1590 | −0.0083* | 0.7512 | 0.2488 |
| 0.0333 | 2.4690 | 0.0106 | 1.2260 | −0.0064 | 0.7769 | 0.2231 | |
| (0.0246) | (0.8758) | (0.0120) | (0.2440) | (0.0039) | (0.1336) | (0.1336) | |
| n = 8 | n = 8 | n = 8 | n = 8 | n = 10 | n = 10 | n = 10 | |
Median, Mean expression, Standard Deviation (SD) and n = Sample sizes are represented. Delta = luciferase activity difference between treated and non-treated transfectants measured in the same experiment. Ratio = relative luciferase activity for treated and non-treated transfectants measured in the same experiment. 1-Ratio = 1 minus relative luciferase activity for treated and non-treated transfectants measured in the same experiment. The statistical analysis was performed using Kruskal-Wallis test followed by the Dunn’s posttest. *P < 0.05 indicating statistical significance compared to G010101a haplotype; #P < 0.05 indicating statistical significance compared to G010101d haplotype.